Analytical Data
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Gene name
HDX
- Application
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Alternative Names
CXorf43 ; D030011N01Rik; FLJ30678; Hdx; HDX_HUMAN; Highly divergent homeobox; MGC126769; MGC126771; OTTHUMP00000062139
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z353
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Expression Region
1-690aa
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AA Sequence
MNLRSVFTVEQQRILQRYYENGMTNQSKNCFQLILQCAQETKLDFSVVRTWVGNKRRKMSSKNSESGTATTGTSLSAPDITVRNVVNIARPSSQQSSWTSANNDVIVTGIYSPASSSSRQGTNKHTDTQITEAHKIPIQKTATKNDTEFQLHIPVQRQVAHCKNASLLLGEKTIILSRQTSVLNAGNSVFNHAKKNYGNSSVQASEMTVPQKPSVCHRPCKIEPVGIQRSYKPEHTGPALHNLCGQKPTIRDPYCRTQNLEIREVFSLAVSDYPQRILGGNAPQKPSSAEGNCLSIAMETGDAEDEYAREEELASMRAQIPSYSRFYESGSSLRAENQSTTLPGPGRNMPNSQMVNIRDMSDNVLYQNRNYHLTPRTSLHTASSTMYSNTNPLRSNFSPHFASSNQLRLSQNQNNYQISGNLTVPWITGCSRKRALQDRTQFSDRDLATLKKYWDNGMTSLGSVCREKIEAVATELNVDCEIVRTWIGNRRRKYRLMGIEVPPPRGGPADFSEQPESGSLSALTPGEEAGPEVGEDNDRNDEVSICLSEGSSQEEPNEVVPNDARAHKEEDHHAVTTDNVKIEIIDDEESDMISNSEVEQVNSFLDYKNEEVKFIENELEIQKQKYFKLQTFVRSLILAMKADDKEQQQALLSDLPPELEEMDFNHASLEPDDTSFSVSSLSEKNVSESL
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Molecular Weight
103.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of HDX (hydrogen-deuterium exchange) recombinant proteins is critical in understanding protein structure, dynamics, and interactions at the molecular level. Protein folding, stability, and functionality are essential aspects of biochemistry, and HDX serves as a powerful technique to probe these characteristics. In HDX experiments, hydrogen atoms in specific regions of the protein are replaced with deuterium, allowing researchers to track the exchange rates of amide protons in peptides. This technique provides insights into protein dynamics, accessibility, and conformational changes in response to various environmental factors. Furthermore, HDX is particularly valuable for studying protein-ligand interactions, as it can reveal binding-induced structural rearrangements and conformational states. With advancements in mass spectrometry, HDX analysis has become more refined, enabling high-resolution mapping of protein regions that participate in binding and interactions. Therefore, research on HDX recombinant proteins offers significant implications for drug design, therapeutic development, and the understanding of various diseases related to protein misfolding or dysfunction. As such, the ongoing exploration of HDX methodologies and their application to recombinant proteins is paramount in the fields of structural biology and biochemistry.
