Analytical Data
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基因名
GALK1
- Application
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别名
GALK1；GALK;Galactokinase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51570
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表达区间
1-392aa
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氨基酸序列
MAALRQPQVAELLAEARRAFREEFGAEPELAVSAPGRVNLIGEHTDYNQG LVLPMALELMTVLVGSPRKDGLVSLLTTSEGADEPQRLQFPLPTAQRSLE PGTPRWANYVKGVIQYYPAAPLPGFSAVVVSSVPLGGGLSSSASLEVATY TFLQQLCPDSGTIAARAQVCQQAEHSFAGMPCGIMDQFISLMGQKGHALL IDCRSLETSLVPLSDPKLAVLITNSNVRHSLASSEYPVRRRQCEEVARAL GKESLREVQLEELEAARDLVSKEGFRRARHVVGEIRRTAQAAAALRRGDY RAFGRLMVESHRSLRDDYEVSCPELDQLVEAALAVPGVYGSRMTGGGFGG CTVTLLEASAAPHAMRHIQEHYGGTATFYLSQAADGAKVLCL
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分子量
42 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GALK1 (Galactokinase 1) is an enzyme that plays a crucial role in the galactose metabolism pathway by catalyzing the conversion of galactose to galactose-1-phosphate, a vital process for energy production and proper cellular function. Mutations in the GALK1 gene can lead to classic galactosemia, a genetic disorder characterized by the accumulation of galactose in the body, resulting in severe health issues including liver damage, cataracts, and cognitive impairment. As a result, studying GALK1 and its recombinant protein is essential for understanding the biochemical mechanisms underlying these disorders. Furthermore, recombinant GALK1 protein can serve as a valuable model for designing therapeutic strategies, including enzyme replacement therapy and gene therapy, aimed at ameliorating the symptoms of galactosemia. The production and characterization of GALK1 recombinant protein can also facilitate the development of high-throughput screening methods for potential inhibitors that can mitigate the effects of the disease. Additionally, understanding the structure and function of the GALK1 protein at a molecular level can provide insights into enzyme regulation and substrate specificity, which are critical for advancements in metabolic engineering and the development of biosensors. Overall, research on GALK1 recombinant protein not only enhances our understanding of galactose metabolism but also paves the way for innovative therapeutic approaches to treat genetic disorders associated with this pathway.
