Analytical Data
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基因名
SRD5a1
- Application
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别名
SRD5a1；3-oxo-5-alpha-steroid 4-dehydrogenase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18405
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表达区间
1-259aa
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氨基酸序列
MATATGVAEERLLAALAYLQCAVGCAVFARNRQTNSVYGRHALPSHRLRV PARAAWVVQELPSLALPLYQYASESAPRLRSAPNCILLAMFLVHYGHRCL IYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDWVTDPR FLIGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYF GEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKF RKIIIPFLF
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分子量
55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SRD5A1 (Steroid 5-alpha-reductase type 1) is an important enzyme that catalyzes the conversion of testosterone into dihydrotestosterone (DHT), a potent androgen involved in the development of male characteristics and various physiological processes. Understanding SRD5A1 is crucial for studying disorders associated with androgen imbalance, such as androgenetic alopecia, prostate hyperplasia, and certain types of cancer. In recent years, research has focused on the recombinant production of SRD5A1 protein to elucidate its molecular mechanisms and regulatory pathways. The ability to produce recombinant SRD5A1 in a controlled environment allows for the exploration of its enzymatic activity, interaction with other proteins, and the impact of genetic variations on its function. Furthermore, these studies can facilitate the development of targeted therapies for conditions linked to altered androgen metabolism. Previous studies have indicated that SRD5A1 may also play a role in diverse biological functions beyond androgen metabolism, including influence on metabolic syndrome and cardiovascular health. Consequently, generating large quantities of high-purity SRD5A1 through recombinant technology not only aids in basic research but also holds potential for therapeutic applications, making it a significant target in endocrinology and pharmacology.
