Analytical Data
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Gene name
rpoS
- Application
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Alternative Names
rpoS；RNA polymerase sigma factor RpoS
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13445
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Expression Region
1-330aa
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AA Sequence
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
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Molecular Weight
38.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RpoS, also known as the sigma factor σ^S, plays a crucial role in the survival and stress response of bacteria, particularly in Escherichia coli. It regulates the expression of genes necessary for adaptation to various environmental stresses, such as nutrient deprivation, oxidative stress, and acid conditions. The study of RpoS and its recombinant protein has gained significant interest due to its implications in bacterial survival, virulence, and persistence under adverse conditions. Understanding the function and regulation of RpoS can provide insights into microbial pathogenesis and resistance mechanisms, making it a potential target for novel antibacterial strategies. Additionally, the recombinant production of RpoS can facilitate the study of its interaction with other proteins and regulatory networks, advancing our knowledge of bacterial stress response pathways. Researchers exploit various expression systems to produce RpoS protein, allowing detailed biochemical and structural studies to elucidate its mechanism of action. Overall, RpoS serves as a significant model for understanding bacterial adaptation in fluctuating environments, highlighting its importance in microbiological research and potential therapeutic developments.
