Analytical Data
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基因名
pht3
- Application
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别名
pht3；Phthalate 4.5-dioxygenase oxygenase subunit
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q05183
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表达区间
1-439aa
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氨基酸序列
MLTPEENLLLCRVEGDAPMGQMMRRHWTPVCLLEEVSEPDGTPVRARLFGEDLVVFRDTDGRVGVMDEYCPHRRVSLIYGRNENSGLRCLYHGWKMDVDGNVVEMVSEPAASNMCQKVKHTAYKTREWGGFVWAYMGPQDAIPEFVPPAWAPHEHVRVSIAKAIIPCNWAQILEGAIDSAHSSSLHSSDFVPARVGGAEATSKNWLRPSTDKAPRMQVERTSYGFRYAALRRPIQNAATSEYVRSTVFVAPATALIPPNNLYNVANINVPIDDTHTAFYFMAWGNPDNTPETETWRKFLGQQVGIDLDDSYRPLRNDGNRFFQDREAMKNGNFTGIKGFPNQDIAMWVTMGPIADRSDERLGASDLAVVEFRRVMLDALAAFQAGESAIGTGEKAIPSRICSFQAIVSKDIDWRDYQARYVWALDDANIVAEPDYEVHT
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分子量
49.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PHT3 is a member of the PHT (phosphate transporter) family, primarily involved in the transportation of phosphate ions in various biological systems. Understanding the role of PHT3 is particularly crucial in the context of plant physiology and nutrient uptake, as phosphate is an essential macronutrient for plant growth and development. Phosphate availability often limits agricultural productivity, making the study of PHT3 and its mechanisms of action vital for improving crop efficiency and resilience in low-phosphate soils. Recent research indicates that PHT3 may also play a significant role in regulating intracellular phosphate homeostasis and signaling pathways associated with nutrient stress. Additionally, the recombinant production of PHT3 proteins allows for in vitro studies to elucidate their structural and functional properties, which can pave the way for genetic engineering strategies aimed at enhancing phosphate utilization in crops. This research holds the potential to contribute to sustainable agriculture practices by developing plants that can thrive in phosphate-deficient environments, ensuring food security in the face of global challenges such as soil degradation and climate change.
