Analytical Data
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Gene name
GTF2I
- Application
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Alternative Names
BAP 135; BAP-135; BAP135; Bruton tyrosine kinase associated protein 135; Bruton tyrosine kinase-associated protein 135; BTK associated protein 135; BTK associated protein 135kD; BTK associated protein; BTK-associated protein 135; BTKAP 1; BTKAP1; DIWS; FLJ38776; FLJ56355; General transcription factor II i; General transcription factor II-I; General transcription factor IIi; GTF 2I; Gtf2i; GTF2I_HUMAN; GTFII I; GTFII-I; IB 291; IB291; SPIN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P78347
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Expression Region
36-274aa
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AA Sequence
ELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGTEMEVPAEG
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Molecular Weight
52.03 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2I (General Transcription Factor II-I) is a crucial transcription factor involved in the regulation of gene expression, playing a significant role in various cellular processes, including cell growth and differentiation. Alterations in GTF2I function have been implicated in several developmental disorders and diseases, particularly the 22q11.2 deletion syndrome, where it is believed to contribute to the pathophysiology of related phenotypes. Research into recombinant GTF2I protein aims to elucidate its structure and function, providing insights into its molecular mechanisms and interactions within the transcriptional machinery. By producing GTF2I in a recombinant form, scientists can investigate its biochemical properties, including its affinity for DNA and interactions with other transcription factors and co-regulators. These studies are crucial for understanding how dysregulation of GTF2I can lead to disease, and they may pave the way for potential therapeutic strategies targeting GTF2I-related pathways. Moreover, recombinant GTF2I can serve as a valuable tool in high-throughput screening assays to identify small molecules or peptides that could modulate its activity, offering hope for developing novel interventions in conditions associated with GTF2I dysfunction. Overall, the research on recombinant GTF2I protein holds promise not only for clarifying fundamental aspects of transcription regulation but also for addressing clinically relevant questions regarding its role in human health and disease.
