Analytical Data
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基因名
gyrB
- Application
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别名
gyrB；acrB；cou；himB；DNA gyrase subunit B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0AES6
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表达区间
2-392aa
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氨基酸序列
SNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
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分子量
50.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The GyrB protein, a crucial component of bacterial DNA gyrase, plays a significant role in DNA supercoiling and is essential for bacterial DNA replication and transcription. As part of a heteromeric complex with GyrA, GyrB facilitates the introduction of negative supercoils into DNA, enabling the compact packing of genetic material and the regulation of gene expression. Research on GyrB has gained importance due to its association with antibiotic resistance; mutations in the gyrase genes can lead to resistance against quinolone antibiotics, which target this enzyme to inhibit bacterial growth. Consequently, understanding the structure, function, and genetic variability of GyrB can provide insights into bacterial physiology and the mechanisms underlying antibiotic resistance. In recent studies, researchers have focused on the recombinant expression of GyrB to elucidate its biochemical properties, interactions with GyrA, and response to various inhibitors. Additionally, characterizing GyrB at the molecular level helps identify potential drug targets for novel antimicrobial therapies. This research not only contributes to the fundamental understanding of bacterial DNA processing but also holds promise for combating resistant bacterial strains through the development of new therapeutic strategies.
