Analytical Data
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基因名
GRLF1
- Application
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别名
ARHGAP35; GAP associated protein p190; Glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF 1; GRF-1; GRF1; GRLF 1; GRLF1; GRLF1_HUMAN; KIAA1722; MGC10745; p190 A; p190-A; P190A; P190A; rat; homolog of; p190ARhoGAP; p190RhoGAP; Rho GAP p190A; Rho GTPase-activating protein 35
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRY4
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表达区间
1-36aa
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氨基酸序列
MEATSRSVHGDVGEVGHFEGMAVCWCPRRGILPGLR
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分子量
29.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GRLF1 (G-rich RNA-binding factor 1) is a transcription factor that plays a crucial role in the regulation of gene expression, particularly in response to environmental stresses. Recent studies have highlighted its significant involvement in various biological processes, such as cellular proliferation, differentiation, and apoptosis. GRLF1 has been associated with the regulation of several key genes involved in stress responses, making it an important focus for researchers seeking to understand the molecular mechanisms underlying stress tolerance in plants and other organisms. Moreover, its potential role in human diseases, including cancer, has prompted investigations into its function and interactions within cellular pathways. To elucidate GRLF1's mechanisms of action, the production of recombinant GRLF1 protein has become essential for in vitro studies, allowing researchers to analyze its binding properties, post-translational modifications, and interactions with other proteins or RNA molecules. By generating and characterizing this recombinant protein, researchers aim to gain insights into GRLF1's multifaceted roles and its potential applications in biotechnology and medicine, ultimately contributing to the development of strategies to enhance stress resistance in crops or to identify new therapeutic targets for disease treatment.
