Analytical Data
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基因名
GPR176
- Application
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别名
GPR176; G-protein coupled receptor 176; HB-954
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14439
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表达区间
1-515aa
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氨基酸序列
MGHNGSWISPNASEPHNASGAEAAGVNRSALGEFGEAQLYRQFTTTVQVVIFIGSLLGNFMVLWSTCRTTVFKSVTNRFIKNLACSGICASLVCVPFDIILSTSPHCCWWIYTMLFCKVVKFLHKVFCSVTILSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLVYNITTVIVPVVVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVMVFILCSVPYATLVVYQTVLNVPDTSVFLLLTAVWLPKVSLLANPVLFLTVNKSVRKCLIGTLVQLHHRYSRRNVVSTGSGMAEASLEPSIRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYIGSADFQAKEIFSTCLEGEQGPQFAPSAPPLSTVDSVSQVAPAAPVEPETFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKVPKVGRVERKMSRNNKVSIFPKVDS
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分子量
83.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPR176, a member of the G protein-coupled receptor (GPCR) family, has garnered attention in recent years due to its potential role in various physiological and pathological processes. GPCRs are crucial for cellular communication and are implicated in numerous signal transduction pathways. Research indicates that GPR176 may be involved in metabolic regulation, and its expression is notably upregulated in certain tissues, suggesting a role in endocrine signaling. Additionally, there is emerging evidence linking GPR176 to neurodegenerative diseases, particularly due to its localization in the brain and roles in modulating neuronal function. The need for recombinant GPR176 protein arises from the desire to elucidate its structure-function relationships, understand its signaling mechanisms, and assess its interactions with potential ligands. Producing a recombinant form of GPR176 allows for detailed biochemical studies, including ligand-binding assays, intracellular signaling assays, and potentially therapeutic targeting. Understanding the biological functions of GPR176 could pave the way for novel approaches in treating metabolic disorders and neurodegenerative conditions, highlighting its significance in both basic research and clinical applications. As such, the development of recombinant GPR176 protein represents a critical step in characterizing this receptor and its implications in health and disease.
