Analytical Data
-
Gene name
Arid5a
- Application
-
Alternative Names
Arid5a；MRF1；AT-rich interactive domain-containing Protein 5A
-
Species
Mouse
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q3U108
-
Expression Region
1-590aa
-
AA Sequence
MAAPPAKGNTEQSEEGDLPQLPVSPKPDDEQSRSQSPTQLQDSPEAGGEQEEEQAFLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHLKGEDDKPLPPTKPRKQYKMAKELRGDDGTTEKLKKAKDSEERRVEQTTPGKTKSDATGQTQLPCQGSSRDSTEQLGPVSGPSPPLTGASSCPEAYKRLLSSFYCKGAHGIMSPLAKKKLLAQVSKAEALQCQEEGCRHGARSPNKDIQDSPQNLRGPAENSEHQLTPREGLQAPGGSTRMEAQVGPCPTAPMFSGCFHAYPTEVLKPVSQHPRDFFSGLKDRVLLGPPGKEEGPTTKESHLVWGGDANHPSAFHKGSTRKRSFYPKPKACWVSPMAKVPTERPGAPSPHPSSPGLGSKRGLEEEGFAHGGKKLRAVSPFLKEVDSKETGGKPAAPGLAVSCLLGPTPGPTPPEAYRGTMLRCPLNFTGSADPLKGQASLPFSPLVIPAFPAHLLATTGSSPMAASLMHFPPTPYDAVLRNRLGPASSAWHMPPVTTYAAPHFFHLNTKL
-
Molecular Weight
65.9 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Arid5a, a member of the AT-rich interactive domain-containing protein family, plays a crucial role in various cellular processes, including transcriptional regulation and chromatin remodeling. Its significance has been increasingly recognized in the context of immune responses and cell differentiation. Studies have shown that Arid5a is involved in the regulation of key immune signaling pathways, particularly those related to the development and function of T cells, making it a potential target for therapeutic interventions in autoimmune diseases and cancer. The ability of Arid5a to modulate gene expression by interacting with DNA and other regulatory proteins underscores its importance in maintaining cellular homeostasis. Additionally, research has indicated that Arid5a may influence the stability and activity of specific mRNA transcripts, highlighting its role in post-transcriptional regulation. Given its multifaceted functions, the recombinant production of Arid5a protein is essential for detailed biochemical characterization and functional studies. Understanding the molecular mechanisms through which Arid5a operates could pave the way for novel strategies in enhancing immune responses or mitigating pathological conditions, thus providing a valuable avenue for future research and therapeutic development.
