Analytical Data
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Gene name
Spp1
- Application
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Alternative Names
Spp1；SPP1；Sphingosine-1-phosphate phosphatase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10451
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Expression Region
17-300aa
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AA Sequence
IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKF
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Molecular Weight
34.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Spp1, also known as secreted phosphoprotein 1 or osteopontin, is a glycoprotein that plays a crucial role in various biological processes, including bone remodeling, immune responses, and cell signaling. It is produced predominantly by osteoblasts and has been shown to be involved in pathophysiological conditions such as cancer metastasis, cardiovascular diseases, and chronic inflammatory diseases. Research on Spp1 has gained traction due to its multifaceted functions; for instance, it serves as a cell adhesion molecule and modulates immune cell functions by interacting with various receptors, including integrins and CD44. The characterization of Spp1 as a biomarker for certain diseases has also sparked significant interest in its therapeutic potential. Recombinant Spp1 proteins are utilized in laboratories to further investigate its roles in cellular processes, elucidate its mechanisms of action, and explore its implications in disease. The development and purification of Spp1 recombinant proteins allow researchers to conduct in vitro and in vivo studies, ultimately aiming to pave the way for novel therapeutic strategies targeting diseases associated with dysregulated Spp1 expression. Consequently, Spp1 continues to be a key focus of research, bridging basic science and clinical applications, and holding promise for improving disease outcomes through targeted interventions.
