Analytical Data
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基因名
EGFR
- Application
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别名
EGFR；ERBB；ERBB1；HER1;Epidermal growth factor receptor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00533
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表达区间
25-645aa
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氨基酸序列
LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNY DLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSN YDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLS NMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCR GKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTT YQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVR KCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPV AFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLE IIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANT INWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCV SCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGR GPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNC TYGCTGPGLEGCPTNGPKIPS—Linker(gggsgggs)—10*His(hhhh hhhhhh)
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分子量
71 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Epidermal growth factor receptor (EGFR) is a critical protein involved in various cellular processes, including proliferation, differentiation, and survival. Its dysfunction, often through mutations or overexpression, is strongly associated with several cancers, particularly non-small cell lung cancer (NSCLC) and head and neck squamous cell carcinoma. Research has increasingly focused on the development of therapeutic strategies targeting EGFR, such as monoclonal antibodies and tyrosine kinase inhibitors, to improve patient outcomes. In order to facilitate these studies, the recombinant expression of EGFR protein has become essential for understanding its structural and functional properties. The production of recombinant EGFR allows researchers to investigate the receptor's signaling pathways, binding interactions, and mechanisms of action in a controlled environment. Moreover, the ability to produce large quantities of pure EGFR protein is crucial for both in vitro and in vivo studies, enabling the testing of novel therapeutic agents and the development of diagnostic tools. Advances in biotechnology, including the use of various expression systems (e.g., bacterial, yeast, insect, and mammalian cells), have further enhanced the efficiency and yield of recombinant EGFR production. Ultimately, a comprehensive understanding of EGFR biology and the development of effective inhibitors could lead to better-targeted therapies, making EGFR a focal point in cancer research and treatment innovation.
