Analytical Data
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Gene name
EGFR
- Application
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Alternative Names
EGFR；ERBB；ERBB1；HER1;Epidermal growth factor receptor
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P00533
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Expression Region
25-645aa
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AA Sequence
LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNY DLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSN YDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLS NMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCR GKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTT YQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVR KCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPV AFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLE IIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANT INWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCV SCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGR GPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNC TYGCTGPGLEGCPTNGPKIPS—Linker(gggsgggs)—10*His(hhhh hhhhhh)
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Molecular Weight
71 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Epidermal growth factor receptor (EGFR) is a critical protein involved in various cellular processes, including proliferation, differentiation, and survival. Its dysfunction, often through mutations or overexpression, is strongly associated with several cancers, particularly non-small cell lung cancer (NSCLC) and head and neck squamous cell carcinoma. Research has increasingly focused on the development of therapeutic strategies targeting EGFR, such as monoclonal antibodies and tyrosine kinase inhibitors, to improve patient outcomes. In order to facilitate these studies, the recombinant expression of EGFR protein has become essential for understanding its structural and functional properties. The production of recombinant EGFR allows researchers to investigate the receptor's signaling pathways, binding interactions, and mechanisms of action in a controlled environment. Moreover, the ability to produce large quantities of pure EGFR protein is crucial for both in vitro and in vivo studies, enabling the testing of novel therapeutic agents and the development of diagnostic tools. Advances in biotechnology, including the use of various expression systems (e.g., bacterial, yeast, insect, and mammalian cells), have further enhanced the efficiency and yield of recombinant EGFR production. Ultimately, a comprehensive understanding of EGFR biology and the development of effective inhibitors could lead to better-targeted therapies, making EGFR a focal point in cancer research and treatment innovation.
