Analytical Data
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Gene name
PDE4D
- Application
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Alternative Names
PDE4D；DPDE3；3'.5'-cyclic-AMP phosphodiesterase 4D
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08499
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Expression Region
2-507aa
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AA Sequence
ASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKE KEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNK WGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLED HYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHD VDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQN LTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLD NYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERG MEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLED NREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSG SQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVID DRSPDT
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Molecular Weight
84 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDE4D (phosphodiesterase 4D) is an enzyme that plays a crucial role in the hydrolysis of cyclic adenosine monophosphate (cAMP), a secondary messenger involved in various physiological processes such as inflammation, cell differentiation, and cell signaling. Aberrant expression or activity of PDE4D has been implicated in several diseases, including cardiovascular disorders, cancer, and neurodegenerative conditions. Research into the recombinant expression of PDE4D has gained traction due to its potential as a therapeutic target; selective inhibition of PDE4D could lead to novel treatments for these conditions by modulating cAMP levels. The production of recombinant PDE4D proteins allows scientists to investigate the enzyme's structure-function relationships, enzymatic kinetics, and regulatory mechanisms in detail. This research also aids in the identification of potent PDE4D inhibitors through high-throughput screening techniques, which may offer greater specificity and fewer side effects compared to broader phosphodiesterase inhibitors. Understanding PDE4D's role at the molecular level is essential in developing drugs that manipulate cAMP signaling pathways, leading to new strategies for treating diseases linked to cAMP dysregulation.
