Analytical Data
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基因名
PDE1A
- Application
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别名
PDE1A；Dual specificity calcium/calmodulin-dependent 3'.5'-cyclic nucleotide phosphodiesterase 1A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54750
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表达区间
1-535aa
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氨基酸序列
MGSSATEIEELENTTFKYLTGEQTEKMWQRLKGILRCLVKQLERGDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQEARTSSQKCEFIHQ
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分子量
64 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDE1A, or phosphodiesterase 1A, is an enzyme that plays a crucial role in the degradation of cyclic nucleotides, specifically cAMP and cGMP, thereby regulating various cellular signaling pathways. Its activity is critical in many physiological processes, including cardiac function, neuronal signaling, and smooth muscle contraction. The dysregulation of PDE1A has been implicated in several pathological conditions, such as heart failure, neurodegenerative disorders, and hypertension, making it an attractive target for therapeutic intervention. Recent studies have focused on recombinant PDE1A proteins to better understand their structure, catalytic mechanisms, and interactions with specific ligands. By using techniques such as molecular cloning and expression in heterologous systems, researchers aim to produce active PDE1A enzymes that can be characterized biochemically and biophysically. This research not only provides insights into the enzyme's role in health and disease but also aids in the identification of selective inhibitors that could lead to novel treatments for conditions associated with PDE1A dysregulation. Overall, the study of recombinant PDE1A proteins is essential for unraveling their functional significance in various biological contexts and for the development of targeted therapeutic strategies.
