Analytical Data
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Gene name
PDE10A
- Application
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Alternative Names
PDE10A；cAMP and cAMP-inhibited cGMP 3'.5'-cyclic phosphodiesterase 10A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y233
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Expression Region
71-779aa
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AA Sequence
MQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGEC NNSLCIFTPPGIKEGKPRLIPAGPITQGTTVSAYVAKSRKTLLVEDILGD ERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFCLSHQ EVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSL LEHIMIYAKNLVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTK EIRFSIEKGIAGQVARTGEVLNIPDAYADPRFNREVDLYTGYTTRNILCM PIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHR IRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIG PFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHA VTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDH PLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAI IATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTK LWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFY NAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSV AQKAAASED
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Molecular Weight
102 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDE10A, or phosphodiesterase 10A, is an enzyme that plays a critical role in the regulation of intracellular signaling pathways by hydrolyzing cyclic nucleotides, specifically cAMP and cGMP. This enzyme is predominantly expressed in the striatum, a key region of the brain involved in motor control, cognition, and emotion regulation. Dysregulation of PDE10A activity has been implicated in various neuropsychiatric disorders, including schizophrenia and Huntington's disease, making it a promising target for therapeutic interventions. Recent research has focused on the development of PDE10A recombinant proteins to better understand its structure, function, and inhibition mechanisms. These recombinant proteins can serve as valuable tools for studying the enzymatic activity of PDE10A and evaluating small molecule inhibitors, potentially aiding drug discovery efforts. By utilizing advanced techniques such as X-ray crystallography and electron microscopy, researchers aim to elucidate the catalytic mechanisms underlying PDE10A's action and its interaction with regulatory proteins. Overall, the study of PDE10A recombinant proteins not only enhances our understanding of its biological significance but also supports the identification of novel therapeutic strategies for treating disorders associated with its dysfunction.
