Analytical Data
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Gene name
ribA
- Application
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Alternative Names
ribA；GTP cyclohydrolase-2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7I7
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Expression Region
1-196aa
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AA Sequence
MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHTPVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK
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Molecular Weight
25.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the speF recombinant protein is rooted in its crucial role in the biosynthesis of polyamines, particularly spermidine and spermine, which are vital for cellular growth and function. SpeF, or S-adenosylmethionine decarboxylase, is an enzyme that catalyzes the decarboxylation of S-adenosylmethionine to produce decarboxylated S-adenosylmethionine, a key intermediate in polyamine synthesis. Polyamines are known to be involved in various cellular processes, including DNA stabilization, protein synthesis, and cell proliferation. Mutations or dysregulation of speF have been linked to multiple pathological conditions, including cancer and neurodegenerative diseases, highlighting the enzyme's potential as a therapeutic target. The production of recombinant speF protein enables researchers to further investigate its enzymatic properties, functions, and interactions within cellular pathways. Understanding the molecular mechanisms by which speF influences polyamine metabolism can provide insights into its role in health and disease, making it a significant focus for biochemical and pharmacological studies.
