Analytical Data
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Gene name
mcr-8
- Application
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Species
Klebsiella pneumoniae
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A2U7XX87
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Expression Region
177-565aa
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Molecular Weight
46.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCR-8, a variant of the carbapenemase enzyme, has garnered significant attention due to its role in bacterial resistance, particularly against last-resort antibiotics such as carbapenems. Originally identified in *Enterobacter* species, MCR-8 represents a part of a growing family of mobile resistance genes that contribute to the alarming rise in multidrug-resistant infections worldwide. The ability of MCR-8 to hydrolyze carbapenems poses a serious challenge in healthcare settings, complicating treatment options for infections caused by resistant strains. Research on MCR-8 is crucial for understanding its biochemical characteristics, mechanisms of resistance, and the genetic factors that facilitate its spread among bacterial populations. Scientists are focusing on the structural biology of MCR-8 to develop inhibitors that can restore the efficacy of existing antibiotics. Additionally, the epidemiological studies map the prevalence of MCR-8 gene across different geographical regions and its transmission dynamics, which is vital for public health strategies aimed at controlling drug-resistant infections. Understanding the origins, mechanisms, and implications of MCR-8 is essential not only for addressing the immediate threats posed by resistant pathogens but also for informing future drug development and antimicrobial stewardship practices.
