Analytical Data
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Gene name
NOX3
- Application
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Alternative Names
NOX3；MOX2；NADPH oxidase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HBY0
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Expression Region
1-568aa
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AA Sequence
MMGCWILNEGLSTILVLSWLGINFYLFIDTFYWYEEEESFHYTRVILGSTLAWARASALCLNFNCMLILIPVSRNLISFIRGTSICCRGPWRRQLDKNLRFHKLVAYGIAVNATIHIVAHFFNLERYHWSQSEEAQGLLAALSKLGNTPNESYLNPVRTFPTNTTTELLRTIAGVTGLVISLALVLIMTSSTEFIRQASYELFWYTHHVFIVFFLSLAIHGTGRIVRGQTQDSLSLHNITFCRDRYAEWQTVAQCPVPQFSGKEPSAWKWILGPVVLYACERIIRFWRFQQEVVITKVVSHPSGVLELHMKKRGFKMAPGQYILVQCPAISSLEWHPFTLTSAPQEDFFSVHIRAAGDWTAALLEAFGAEGQALQEPWSLPRLAVDGPFGTALTDVFHYPVCVCVAAGIGVTPFAALLKSIWYKCSEAQTPLKLSKVYFYWICRDARAFEWFADLLLSLETRMSEQGKTHFLSYHIFLTGWDENQALHIALHWDENTDVITGLKQKTFYGRPNWNNEFKQIAYNHPSSSIGVFFCGPKALSRTLQKMCHLYSSADPRGVHFYYNKESF
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Molecular Weight
64.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NOX3 (NADPH oxidase 3) is a member of the NOX family of enzymes, which are known to generate reactive oxygen species (ROS) as part of their normal physiological functions. Initially identified in the inner ear, NOX3 has been implicated in various cellular processes, including cell signaling, proliferation, and differentiation. Its role in the auditory system has led to significant interest in understanding NOX3’s function and mechanisms, especially given its association with hearing disorders. Research indicates that NOX3 participates in the regulation of oxidative stress and inflammation, which are crucial in maintaining cellular homeostasis. Furthermore, alterations in NOX3 expression have been linked to pathophysiological conditions such as cancer, neurodegenerative diseases, and cardiovascular disorders. The study of NOX3 recombinant protein offers insights into its enzymatic activity, regulation, and potential therapeutic targets. By understanding the structural and functional characteristics of NOX3, researchers aim to develop strategies to manipulate its activity, potentially leading to innovative treatments for conditions related to oxidative damage and impaired ROS signaling. The recombinant production of NOX3 facilitates detailed biochemical analyses, paving the way for discoveries that could enhance our knowledge of its biological roles and therapeutic potentials in various diseases. Thus, NOX3 stands out as a significant focus in oxidative biology, meriting further exploration to elucidate its contributions to health and disease.
