Cat: PA2000-3678

Recombinant Human pdh Protein,His

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Analytical Data

  • Gene name

    pdh

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    pdh;PHD finger Protein 23

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P08559

  • Expression Region

    31-390aa

  • AA Sequence

    ANDATFEIKK CDLHRLEEGP PVTTVLTRED GLKYYRMMQT VRRMELKADQ LYKQKIIRGF CHLCDGQEAC CVGLEAGINP TDHLITAYRA HGFTFTRGLS VREILAELTG RKGGCAKGKG GSMHMYAKNF YGGNGIVGAQ VPLGAGIALA CKYNGKDEVC LTLYGDGAAN QGQIFEAYNM AALWKLPCIF ICENNRYGMG TSVERAAAST DYYKRGDFIP GLRVDGMDIL CVREATRFAA AYCRSGKGPI LMELQTYRYH GHSMSDPGVS YRTREEIQEV RSKSDPIMLL KDRMVNSNLA SVEELKEIDV EVRKEIEDAA QFATADPEPP LEELGYHIYS SDPPFEVRGA NQWIKFKSVS

  • Molecular Weight

    43.2 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

Phosphoenolpyruvate-dependent phosphotransferase system (PTS) plays a crucial role in bacterial carbohydrate transport and metabolism, with phosphoenolpyruvate (PEP) acting as the primary energy source for this process. Among the various proteins involved in this system, the phosphotransferase protein, known as Pdh (phosphoenolpyruvate-dependent dihydroxyacetone kinase), has garnered significant attention in biochemical research. Pdh is essential for the efficient conversion of PEP into various metabolic intermediates, thereby influencing cellular energy levels and substrate utilization. The study of Pdh and its recombinant forms has implications for understanding bacterial growth patterns, metabolism regulation, and enzyme kinetics. Furthermore, recombinant Pdh can be harnessed for biotechnological applications, including the development of biosensors and metabolic engineering strategies aimed at optimizing biofuel production and enhancing industrial fermentation processes. Investigating the structural and functional characteristics of recombinant Pdh proteins can provide insights into their catalytic mechanisms and regulatory roles, which may lead to novel therapeutic approaches against bacterial infections and the enhancement of beneficial microbial strains in agricultural and environmental contexts. Overall, research on recombinant Pdh proteins continues to expand our understanding of bacterial metabolism and presents new avenues for innovation in biotechnology.

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