Analytical Data
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基因名
KCNJ5
- Application
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别名
KCNJ5；GIRK4；G Protein-activated inward rectifier potassium channel 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48544
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表达区间
1-419aa
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氨基酸序列
MAGDSRNAMNQDMEIGVTPWDPKKIPKQARDYVPIATDRTRLLAEGKKPRQRYMEKSGKCNVHHGNVQETYRYLSDLFTTLVDLKWRFNLLVFTMVYTVTWLFFGFIWWLIAYIRGDLDHVGDQEWIPCVENLSGFVSAFLFSIETETTIGYGFRVITEKCPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISMRDEKLCLMFRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEINQKSPFWEMSQAQLHQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLEKGFYEVDYNTFHDTYETNTPSCCAKELAEMKREGRLLQYLPSPPLLGGCAEAGLDAEAEQNEEDEPKGLGGSREARGSV
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分子量
47.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KCNJ5, a member of the inwardly rectifying potassium channel family, plays a crucial role in maintaining cellular excitability and fluid balance. Mutations in the KCNJ5 gene have been linked to various pathophysiological conditions, particularly primary aldosteronism, a form of hypertension characterized by excess aldosterone production. These mutations often lead to altered channel function, which can result in hyperpolarization of cell membranes and subsequent effects on hormone secretion. Given its significance in cardiovascular health and potential as a therapeutic target, researchers have increasingly focused on the characterization and functional analysis of KCNJ5 recombinant proteins. By expressing and purifying KCNJ5 in heterologous systems, scientists aim to elucidate its mechanisms of action, the impact of specific mutations, and the channel's interaction with other cellular proteins. This knowledge is essential for understanding the role of KCNJ5 in health and disease and could inform the development of new treatment strategies for conditions linked to potassium channel dysfunction. The study of KCNJ5 recombinant proteins is thus pivotal in both basic and translational research, providing insights that could lead to novel interventions for hypertension and related disorders.
