Analytical Data
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基因名
mpt63
- Application
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别名
mpt63；Immunogenic Protein MPT63
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P9WIP0
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表达区间
30-159aa
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氨基酸序列
AYPITGKLGSELTMTDTVGQVVLGWKVSDLKSSTAVIPGYPVAGQVWEATATVNAIRGSVTPAVSQFNARTADGINYRVLWQAAGPDTISGATIPQGEQSTGKIYFDVTGPSPTIVAMNNGMEDLLIWEP
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分子量
17.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MPT63, a recombinant protein derived from Mycobacterium tuberculosis, has garnered significant attention in the field of tuberculosis (TB) research due to its potential role in immunodiagnosis and vaccine development. This protein is part of the ESAT-6/CFP-10 family, which is known to be involved in the pathogenesis of TB. Previous studies have indicated that MPT63 elicits strong immune responses, making it a candidate for differentiating between active TB and latent infections. Additionally, MPT63's immunogenic properties have led researchers to explore its application in various diagnostic assays, such as interferon-gamma release assays (IGRAs), which are crucial for identifying individuals infected with the bacterium. The study of MPT63 is particularly vital in regions with high TB prevalence, where effective and rapid diagnostic tools are needed to manage this public health challenge. Furthermore, the recombinant nature of MPT63 allows for consistent production and scalability, making it a practical choice for further research. Overall, understanding the role of MPT63 in the immune response to Mycobacterium tuberculosis may lead to advancements in TB diagnostics and therapeutics, contributing to global efforts in controlling and ultimately eradicating tuberculosis.
