Analytical Data
-
Gene name
mpt63
- Application
-
Alternative Names
mpt63；Immunogenic Protein MPT63
-
Species
E.coli
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P9WIP0
-
Expression Region
30-159aa
-
AA Sequence
AYPITGKLGSELTMTDTVGQVVLGWKVSDLKSSTAVIPGYPVAGQVWEATATVNAIRGSVTPAVSQFNARTADGINYRVLWQAAGPDTISGATIPQGEQSTGKIYFDVTGPSPTIVAMNNGMEDLLIWEP
-
Molecular Weight
17.7 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MPT63, a recombinant protein derived from Mycobacterium tuberculosis, has garnered significant attention in the field of tuberculosis (TB) research due to its potential role in immunodiagnosis and vaccine development. This protein is part of the ESAT-6/CFP-10 family, which is known to be involved in the pathogenesis of TB. Previous studies have indicated that MPT63 elicits strong immune responses, making it a candidate for differentiating between active TB and latent infections. Additionally, MPT63's immunogenic properties have led researchers to explore its application in various diagnostic assays, such as interferon-gamma release assays (IGRAs), which are crucial for identifying individuals infected with the bacterium. The study of MPT63 is particularly vital in regions with high TB prevalence, where effective and rapid diagnostic tools are needed to manage this public health challenge. Furthermore, the recombinant nature of MPT63 allows for consistent production and scalability, making it a practical choice for further research. Overall, understanding the role of MPT63 in the immune response to Mycobacterium tuberculosis may lead to advancements in TB diagnostics and therapeutics, contributing to global efforts in controlling and ultimately eradicating tuberculosis.
