Cat: PA2000-3667

Recombinant E.coli APX2 Protein,His

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Analytical Data

  • Gene name

    APX2

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    APX2;SS622;L-ascorbate peroxidase 2. cytosolic

  • Species

    E.coli

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q1PER6

  • Expression Region

    4-250aa

  • AA Sequence

    KSYPEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELGFADK

  • Molecular Weight

    31.5 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

APX2 (Ascorbate Peroxidase 2) is a crucial enzyme involved in the detoxification of hydrogen peroxide in plants, playing a significant role in their response to oxidative stress. This enzyme is part of the ascorbate-glutathione cycle, which is essential for maintaining cellular redox balance and protecting against oxidative damage. Research on APX2 has gained prominence due to its implications in enhancing plant stress tolerance, improving crop yield, and understanding plant resilience mechanisms under adverse environmental conditions. With increasing concerns about climate change and its impact on agriculture, APX2 is of particular interest for genetic engineering and crop improvement strategies. The study of APX2 recombinant proteins has facilitated a deeper understanding of its structure-function relationships, catalytic mechanisms, and interaction with other protective pathways in plants. Additionally, the expression of APX2 in model organisms has provided valuable insights into its physiological roles and potential applications in biotechnological advancements aimed at developing stress-resistant crops. Overall, the investigation of APX2 recombinant proteins not only contributes to fundamental plant biology but also holds potential for practical applications in sustainable agriculture.

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