Analytical Data
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Gene name
pyrF
- Application
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Alternative Names
pyrF；Orotidine 5'-phosphate decarboxylase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
C4ZTX6
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Expression Region
1-245aa
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AA Sequence
MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLKVGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASGGARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQKCGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYMVIGRPVTQSVDPAQTLKAINASLQRSA
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Molecular Weight
33.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The pyrF gene, encoding orotidine 5'-monophosphate decarboxylase (OMPD), plays a pivotal role in the de novo pyrimidine biosynthesis pathway, specifically in the conversion of orotidine monophosphate (OMP) to uridine monophosphate (UMP). This enzymatic step is crucial for the synthesis of nucleotides, which are fundamental for DNA and RNA production. Researchers have become increasingly interested in the pyrF gene and its protein product due to its potential applications in biotechnology and medicine. The study of pyrF recombinants can enhance our understanding of nucleotide metabolism and enable the development of novel biosynthetic pathways for producing pyrimidine derivatives. Additionally, OMPD serves as a target for antimicrobial drug design, as inhibitors of this enzyme could disrupt nucleotide synthesis in pathogenic organisms. Recent advances in recombinant DNA technology allow for the functional expression and purification of pyrF protein, facilitating detailed structural and functional analyses. This work not only provides insights into the enzyme's catalytic mechanism but also explores its evolutionary significance and potential interactions with other metabolic pathways. Overall, the research on pyrF recombinants is not only academically significant but also holds promise for practical applications in therapeutic developments and synthetic biology.
