Analytical Data
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基因名
DHPS
- Application
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别名
DHPS；DS;Deoxyhypusine synthase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49366
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表达区间
1-369aa
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氨基酸序列
MEGSLEREAP AGALAAVLKH SSTLPPESTQ VRGYDFNRGV NYRALLEAFG TTGFQATNFG RAVQQVNAMI EKKLEPLSQD EDQHADLTQS RRPLTSCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE NGINRIGNLL VPNENYCKFE DWLMPILDQM VMEQNTEGVK WTPSKMIARL GKEINNPESV YYWAQKNHIP VFSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKCTGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RVDAQPVKVY ADASLVFPLL VAETFAQKMD AFMHEKNED
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Dihydropteroate synthase (DHPS) is a pivotal enzyme in the folate biosynthesis pathway, catalyzing the condensation of para-aminobenzoate (PABA) with p-hydroxybenzoate to form dihydropteroate. This enzyme is notably absent in mammals, making it a prime target for antimicrobial drug development, particularly against bacterial infections. The significance of DHPS is underscored by its role in conferring resistance to sulfonamide antibiotics, which inhibit this enzyme. The emergence of resistant strains has prompted extensive research into the structure and function of DHPS from various pathogens, aiming to unravel the mechanisms of resistance and identify potential inhibitors. Recombinant DHPS proteins have been expressed and purified for kinetic and structural studies, providing insights into enzyme regulation and substrate interactions. Furthermore, advancements in genetic engineering techniques have facilitated the exploration of DHPS variants, enabling the investigation of how mutations affect enzyme activity and susceptibility to inhibitors. Understanding the intricate details of DHPS at the molecular level can pave the way for novel therapeutic strategies, including the design of more effective inhibitors that can circumvent existing resistance mechanisms. Overall, the study of recombinant DHPS not only enhances our fundamental knowledge of microbial metabolism but also serves as a vital component in combating antibiotic resistance, thereby contributing to global health initiatives.
