Analytical Data
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Gene name
GKRP
- Application
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Alternative Names
GKRP；Glucokinase regulatory Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14397
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Expression Region
1-625aa
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AA Sequence
MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIV RLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASRE GTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIG PEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFE RAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECI HTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSL TEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLK KLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKI LQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSD DIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEA VRSALAGPGQKRTADPLEILEPDVQ
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Molecular Weight
85 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GKRP (Glucokinase Regulatory Protein) is a significant regulatory protein involved in glucose metabolism, acting primarily in the liver. It plays a critical role in the modulation of glucokinase, an enzyme responsible for converting glucose to glucose-6-phosphate, thereby influencing insulin secretion and glucose homeostasis. The regulation of GKRP is vital in understanding metabolic disorders, particularly type 2 diabetes and obesity, as its dysfunction can lead to impaired glucose sensing and regulation. Research has increasingly focused on recombinant GKRP as a potential therapeutic target to enhance glucose metabolism and improve metabolic health. By studying GKRP's structure, function, and interactions within metabolic pathways, scientists aim to develop novel strategies for managing glucose-related diseases. Recent advances in recombinant protein technology have enabled the production of GKRP in sufficient quantities for detailed study, revealing insights into its regulatory mechanisms and potential as a drug target. Understanding GKRP's role in liver function and overall metabolism could pave the way for innovative treatments aimed at restoring metabolic balance in individuals suffering from dysregulated glucose homeostasis.
