Analytical Data
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Gene name
DbH
- Application
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Alternative Names
DbH；MOX;DBH-like monooxygenase Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09172
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Expression Region
40-617aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMSAPRESPLPYHIPLDPEGSLELSWNVSYT QEAIHFQLLVRRLKAGVLFGMSDRGELENADLVVLWTDGDTAYFADAWSD QKGQIHLDPQQDYQLLQVQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHL VYGILEEPFRSLEAINGSGLQMGLQRVQLLKPNIPEPELPSDACTMEVQA PNIQIPSQETTYWCYIKELPKGFSRHHIIKYEPIVTKGNEALVHHMEVFQ CAPEMDSVPHFSGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLA FGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELG LVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGR KVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTY NTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHL INRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKS SAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG
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Molecular Weight
67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DbH (Dopamine β-hydroxylase) is an enzyme critical for the biosynthesis of norepinephrine from dopamine, playing a vital role in the physiological regulation of the autonomic nervous system and the stress response. Research on DbH has gained traction due to its implications in various neurovascular disorders and potential therapeutic applications in treating conditions like hypertension, heart failure, and mood disorders. This enzyme's unusual structure and functioning, particularly its ability to generate both norepinephrine and epinephrine, have made it a focal point in biochemistry and pharmacology. Investigating DbH not only enhances our understanding of catecholamine metabolism but also aids in the development of drugs targeting neurotransmitter systems. Furthermore, the recombinant form of DbH offers a powerful tool for studying its activity and generating potential inhibitors for clinical applications. As such, DbH research holds promise for advancing our knowledge of neurochemistry, developing novel therapeutic strategies, and addressing critical health issues related to catecholamine dysregulation.
