Analytical Data
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Gene name
DAO
- Application
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Alternative Names
DAO；DAMOX;D-amino-acid oxidase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P14920
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Expression Region
1-347aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMRVVVIGAGVIGLSTALCIHERYHSVLQPL DIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVH SPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGY GWFHTSLILEGKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVN CTGVWAGALQRDPLLQPGRGQIMKVDAPWMKHFILTHDPERGIYNSPYII PGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERT GFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLF GRILEEKKLSRMPPSHL
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Molecular Weight
42 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Dao (D-amino acid oxidase) is an enzyme that plays a crucial role in the metabolism of D-amino acids, which are not commonly found in proteins but can influence various physiological processes. In recent years, research has increasingly focused on the role of D-amino acids in neurological functions, cellular signaling, and even the development of specific diseases. The study of DAO has gained significant momentum due to its potential implications in conditions such as schizophrenia, where altered levels of D-serine, a substrate of DAO, have been implicated. Additionally, the enzyme is of interest in the context of cellular stress responses and metabolic disorders. Recent advances in protein engineering have enabled the development of recombinant DAO proteins with enhanced stability, activity, and specificity, opening new avenues for therapeutic applications and biochemical studies. These restructured proteins can be utilized to better understand the enzyme's mechanism, its interaction with substrates, and its regulatory pathways. As research continues, unraveling the functional roles and potential therapeutic benefits of DAO and its products is becoming increasingly important in the fields of biochemistry, pharmacology, and neuroscience, highlighting the need for innovative techniques to study and manipulate this enzyme systematically.
