Analytical Data
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基因名
CYP2E1
- Application
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别名
CYP2E1；CYP2E;Cytochrome P450 2E1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P05181
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表达区间
29-493aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS
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分子量
56 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CYP2E1, a member of the cytochrome P450 superfamily, plays a crucial role in the metabolism of various xenobiotics, including drugs and environmental toxins. It is predominantly expressed in the liver and is known for its ability to oxidize low-molecular-weight substrates, leading to both detoxification and bioactivation of compounds. Research on CYP2E1 has gained significant attention due to its involvement in the metabolic activation of procarcinogens, which can result in the formation of reactive metabolites and contribute to the development of diseases such as cancer and liver damage. Additionally, CYP2E1 is implicated in ethanol metabolism and is induced under conditions of oxidative stress, further highlighting its importance in metabolic pathways and disease mechanisms. The production of recombinant CYP2E1 protein allows for in-depth studies of its enzymatic functions, substrate specificity, and interaction with various inhibitors and inducers. By understanding the structural and functional characteristics of CYP2E1, researchers can investigate its role in pharmacogenomics and personalized medicine, aiming to predict individual responses to therapeutics based on CYP2E1 activity. Overall, the study of CYP2E1 recombinant protein is essential for elucidating its physiological and pathological roles, with potential implications for drug development and toxicology.
