Cat: PA1000-9414

Recombinant Human SLC30A8 Protein,His

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Analytical Data

  • Gene name

    SLC30A8

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    SLC30A8;ZNT8;Proton-coupled zinc antiporter SLC30A8

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8IWU4

  • Expression Region

    1-369aa

  • AA Sequence

    MEFLERTYLV NDKAAKMYAF TLESVELQQK PVNKDQCPRE RPEELESGGM YHCHSGSKPT EKGANEYAYA KWKLCSASAI CFIFMIAEVV GGHIAGSLAV VTDAAHLLID LTSFLLSLFS LWLSSKPPSK RLTFGWHRAE ILGALLSILC IWVVTGVLVY LACERLLYPD YQIQATVMII VSSCAVAANI VLTVVLHQRC LGHNHKEVQA NASVRAAFVH ALGDLFQSIS VLISALIIYF KPEYKIADPI CTFIFSILVL ASTITILKDF SILLMEGVPK SLNYSGVKEL ILAVDGVLSV HSLHIWSLTM NQVILSAHVA TAASRDSQVV RREIAKALSK SFTMHSLTIQ MESPVDQDPD CLFCEDPCD

  • Molecular Weight

    44 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

SLC30A8, also known as zinc transporter 8 (ZnT8), is a gene that encodes a protein responsible for regulating cellular zinc levels, particularly in pancreatic beta cells. Its importance has been highlighted by genetic studies linking variants of SLC30A8 with an increased risk of type 2 diabetes. The protein functions to transport zinc ions into the insulin granules, playing a crucial role in insulin crystallization and secretion. Given that zinc is critical for insulin stability and function, understanding the mechanisms of ZnT8 can provide valuable insights into the pathophysiology of diabetes. The study of SLC30A8 recombinant protein seeks to elucidate its structure, function, and interaction with other cellular components, thereby enabling the development of targeted therapeutic strategies to improve insulin secretion and glucose homeostasis. Furthermore, investigating the functional differences between various SLC30A8 variants may help identify potential molecular targets for intervention in diabetes treatment. As such, SLC30A8 presents a significant focus for research aimed at combating the global diabetes epidemic by enhancing our understanding of zinc's role in metabolic health.

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