Analytical Data
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基因名
SPT14
- Application
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别名
(GPI-GlcNAc transferase complex subunit GPI3)(GPI-GnT subunit GPI3)(GlcNAc-PI synthesis protein)
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种属
Saccharomyces cerevisiae
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P32363
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表达区间
1-377aa
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分子量
49.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPT14, also known as Suppressor of Ty 14, is a crucial protein involved in the regulation of gene expression and chromatin organization in various eukaryotic organisms. The protein is part of the SPT (suppressor of Ty) complex, which plays a significant role in maintaining transcriptional integrity by preventing transcriptional interference from neighboring genomic elements, particularly retrotransposons. Research on SPT14 is particularly significant due to its implications in the fundamental understanding of transcription regulation, growth, and development in cells. In eukaryotes, the intricate balance of gene expression is vital for proper cellular function, and disruptions in this balance can lead to various diseases, including cancer. Therefore, the isolation and characterization of SPT14 as a recombinant protein are essential for elucidating its structural and functional properties, understanding its interactions with other proteins, and exploring its role in chromatin dynamics. By studying SPT14 in vitro, researchers aim to develop targeted therapeutic strategies that can manipulate gene expression in a controlled manner, ideally leading to innovative treatments for genetic disorders and cancers where transcription regulation is compromised. Moreover, the insights gained from SPT14 research can contribute to synthetic biology, where harnessing the mechanisms of gene regulation could enable the creation of engineered organisms with desirable traits. Overall, the study of SPT14 not only enhances our understanding of molecular biology but also holds promise for advancing biomedical research and therapeutic applications.
