Analytical Data
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Gene name
CMPK1
- Application
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Alternative Names
CMPK1；CMK；CMPK；UCK;UMP-CMP kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30085
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Expression Region
1-228aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MLSRCRSGLL HVLGLSFLLQ TRRPILLCSP RLMKPLVVFV LGGPGAGKGT QCARIVEKYG YTHLSAGELL RDERKNPDSQ YGELIEKYIK EGKIVPVEIT ISLLKREMDQ TMAANAQKNK FLIDGFPRNQ DNLQGWNKTM DGKADVSFVL FFDCNNEICI ERCLERGKSS GRSDDNRESL EKRIQTYLQS TKPIIDLYEE MGKVKKIDAS KSVDEVFDEV VQIFDKEG
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Molecular Weight
28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CMPK1 (cytidine monophosphate kinase 1) is an essential enzyme in nucleotide metabolism, playing a critical role in the phosphorylation of nucleoside monophosphates, particularly cytidine monophosphate (CMP), into nucleoside diphosphates. This function is vital for DNA and RNA synthesis, cellular energy homeostasis, and regulating various metabolic pathways. Aberrations in CMPK1 activity have been linked to several pathological conditions, including cancer, viral infections, and neurodegenerative diseases. Given its importance, CMPK1 has garnered attention as a potential target for therapeutic intervention. Recent studies have focused on the recombinant expression of CMPK1 to better understand its structure-function relationships, enzymatic mechanisms, and regulatory properties. Utilizing recombinant CMPK1 enables researchers to investigate its kinetic parameters, substrate specificity, and interactions with various ligands, providing insights into its role in cellular metabolism and disease. Moreover, recombinant CMPK1 can serve as a valuable tool in drug discovery and development, facilitating the screening of small molecule inhibitors or activators that could modulate its activity for therapeutic benefit. Understanding the biochemical characteristics of CMPK1 through recombinant protein studies is crucial for developing strategies to manipulate its function in disease contexts, highlighting its potential as a novel drug target.
