Analytical Data
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Gene name
SGPP1
- Application
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Alternative Names
SGPP1；SPP1；Sphingosine-1-phosphate phosphatase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BX95
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Expression Region
1-441aa
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AA Sequence
MSLRQRLAQLVGRLQDPQKVARFQRLCGVEAPPRRSADRREDEKAEAPLAGDPRLRGRQPGAPGGPQPPGSDRNQCPAKPDGGGAPNGVRNGLAAELGPASPRRAGALRRNSLTGEEGQLARVSNWPLYCLFCFGTELGNELFYILFFPFWIWNLDPLVGRRLVVIWVLVMYLGQCTKDIIRWPRPASPPVVKLEVFYNSEYSMPSTHAMSGTAIPISMVLLTYGRWQYPLIYGLILIPCWCSLVCLSRIYMGMHSILDIIAGFLYTILILAVFYPFVDLIDNFNQTHKYAPFIIIGLHLALGIFSFTLDTWSTSRGDTAEILGSGAGIACGSHVTYNMGLVLDPSLDTLPLAGPPITVTLFGKAILRILIGMVFVLIIRDVMKKITIPLACKIFNIPCDDIRKARQHMEVELPYRYITYGMVGFSITFFVPYIFFFIGIS
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Molecular Weight
49.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SGPP1 (sphingosine-1-phosphate phosphatase 1) is an important enzyme involved in the regulation of sphingolipid metabolism, specifically in the degradation of sphingosine-1-phosphate (S1P), a bioactive lipid that plays crucial roles in various physiological processes, including cell proliferation, survival, migration, and immune response. Dysregulation of S1P levels has been implicated in several diseases, such as cancer, cardiovascular disorders, and neurodegenerative conditions. The study of SGPP1 and its recombinant protein form has gained significant attention due to its potential therapeutic implications. By producing recombinant SGPP1, researchers aim to better understand its enzymatic properties, substrate specificity, and regulatory mechanisms, as well as to explore its role in different biological contexts. Furthermore, elucidating the structure-function relationships of SGPP1 can facilitate the development of novel therapeutic strategies targeting sphingolipid signaling pathways. In recent years, advances in recombinant DNA technology and protein expression systems have enabled the successful production and purification of SGPP1, paving the way for comprehensive functional studies and high-throughput screening for small molecule inhibitors. Overall, research on SGPP1 recombinant protein is vital for revealing its biochemical functions and therapeutic potentials, thus contributing to the broader understanding of sphingolipid biology and its implications in health and disease.
