Analytical Data
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Gene name
CFLAR
- Application
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Alternative Names
CFLAR；CASH；CASP8AP1；CLARP；CASP8 and FADD-like apoptosis regulator
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15519
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Expression Region
1-480aa
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AA Sequence
MSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKL SVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVLMAEI GEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLD LLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNN FRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKP LGICLIIDCIGNETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMP EHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAG KPKMFFIQNYVVSEGQLENSSLLEVDGPAMKNVEFKAQKRGLCTVHREAD FFWSLCTADMSLLEQSHSSPSLYLQCLSQKLRQERKRPLLDLHIELNGYM YDWNSRVSAKEKYYVWLQHTLRKKLILSYT
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Molecular Weight
55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CFLAR (CASP8 and FADD-like apoptosis regulator) is a significant protein that plays a crucial role in regulating apoptosis and cellular survival. Research indicates that CFLAR functions as an essential inhibitor of caspase-8, thereby modulating cell death pathways and contributing to cancer development and progression. Elevated levels of CFLAR have been associated with various malignancies, suggesting its potential role as a biomarker for cancer prognosis and as a therapeutic target. Understanding the molecular mechanisms behind CFLAR's action is vital, as it may offer insights into the challenges of cancer treatment, particularly in overcoming resistance to apoptosis. Recent studies have focused on the structural aspects of CFLAR, revealing insights into its interactions with other apoptotic factors and identifying domains critical for its function. The development of CFLAR recombinant proteins aims to facilitate further analysis of its biological activities, cellular interactions, and potential as a target for innovative cancer therapies. This research could pave the way for designing new strategies to manipulate apoptotic pathways in cancer cells, ultimately contributing to improved therapeutic outcomes and patient survival.
