Analytical Data
-
基因名
CERT
- Application
-
别名
CERT；CERT；COL4A3BP；STARD11；Ceramide transfer Protein
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9Y5P4-2
-
表达区间
347-598aa
-
氨基酸序列
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF
-
分子量
33 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CERT (Ceramide Transporter) is a crucial protein involved in the intracellular transport of ceramides, which are vital sphingolipids implicated in various cellular processes, including signaling, apoptosis, and membrane structure. The study of CERT has gained interest due to its role in lipid metabolism and its potential implications in diseases such as cancer, diabetes, and neurodegenerative disorders. Researchers have focused on understanding the molecular mechanisms of CERT functioning, including its structure, transport mechanisms, and interactions with other proteins and organelles. The reconstitution of CERT into lipid bilayers or membrane systems has become a pivotal approach for elucidating its functional properties and regulatory pathways. Additionally, by employing techniques such as X-ray crystallography, cryo-electron microscopy, and biophysical assays, scientists aim to uncover the conformational dynamics of CERT and its lipid-binding affinities. This research not only enhances our understanding of ceramide transport but also opens avenues for therapeutic interventions targeting lipid metabolic disorders. Overall, the ongoing studies on CERT and its reconstitution are vital for advancing our knowledge in membrane biology and developing novel strategies for treating related diseases.
