Analytical Data
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Gene name
BIGH3
- Application
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Alternative Names
BIGH3；BIGH3；Transforming growth factor-beta-induced Protein ig-h3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15582
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Expression Region
24-683aa
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AA Sequence
GPAKSPY QLVLQHSRLR GRQHGPNVCA VQKVIGTNRK YFTNCKQWYQ RKICGKSTVI SYECCPGYEK VPGEKGCPAA LPLSNLYETL GVVGSTTTQL YTDRTEKLRP EMEGPGSFTI FAPSNEAWAS LPAEVLDSLV SNVNIELLNA LRYHMVGRRV LTDELKHGMT LTSMYQNSNI QIHHYPNGIV TVNCARLLKA DHHATNGVVH LIDKVISTIT NNIQQIIEIE DTFETLRAAV AASGLNTMLE GNGQYTLLAP TNEAFEKIPS ETLNRILGDP EALRDLLNNH ILKSAMCAEA IVAGLSVETL EGTTLEVGCS GDMLTINGKA IISNKDILAT NGVIHYIDEL LIPDSAKTLF ELAAESDVST AIDLFRQAGL GNHLSGSERL TLLAPLNSVF KDGTPPIDAH TRNLLRNHII KDQLASKYLY HGQTLETLGG KKLRVFVYRN SLCIENSCIA AHDKRGRYGT LFTMDRVLTP PMGTVMDVLK GDNRFSMLVA AIQSAGLTET LNREGVYTVF APTNEAFRAL PPRERSRLLG DAKELANILK YHIGDEILVS GGIGALVRLK SLQGDKLEVS LKNNVVSVNK EPVAEPDIMA TNGVVHVITN VLQPPANRPQ ERGDELADSA LEIFKQASAF SRASQRSVRL APVYQKLLER MKH
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BIGH3, also known as BIGH3 protein or transforming growth factor-beta induced protein, is a member of the thrombospondin family and plays a crucial role in various biological processes, including cell adhesion, proliferation, and differentiation. It is primarily expressed in connective tissues and has been implicated in several ocular diseases, such as corneal dystrophies and wound healing processes. Understanding the mechanisms and functions of BIGH3 is vital for developing therapeutic strategies for these conditions. Research on BIGH3 recombinant protein focuses on elucidating its structural characteristics and biological activities, enabling scientists to explore its potential as a biomarker for disease progression or as a target for therapeutic intervention. Additionally, as a secreted extracellular matrix protein, BIGH3 influences cellular responses to environmental cues, making it a significant factor in tissue remodeling and fibrosis. Recent advances in recombinant protein technologies have facilitated the production and purification of BIGH3, allowing for detailed studies of its interactions with other proteins and cellular components. This research not only enhances our comprehension of BIGH3's role in health and disease but also holds promise for innovative approaches to treat or prevent conditions associated with its dysregulation. Ultimately, the ongoing investigation into BIGH3 recombinant protein offers valuable insights that could lead to improved clinical outcomes in various pathologies, particularly those affecting the eye and connective tissue systems.
