Analytical Data
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Gene name
Sp1
- Application
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Alternative Names
Sp1；TSFP1；Transcription factor Sp1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10645
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Expression Region
19-457aa
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AA Sequence
LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG
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Molecular Weight
50.9kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sp1 is a crucial transcription factor that plays a significant role in the regulation of various genes involved in cellular processes such as growth, differentiation, and apoptosis. Its function is essential in both normal physiology and disease states, including cancer. The interest in Sp1 research stems from its involvement in the transcriptional regulation of genes associated with oncogenesis and tumor progression, which has implications for targeted therapies and biomarkers in cancer treatment. Furthermore, the protein's ability to interact with various co-factors and respond to different signaling pathways makes it a complex yet promising target for therapeutic intervention. Studies have shown that abnormal expression of Sp1 is linked to various malignancies, prompting researchers to investigate its potential as a diagnostic and prognostic marker. The development and characterization of recombinant Sp1 protein allow for in-depth studies of its structural properties and functional mechanisms, enhancing our understanding of its role in gene regulation. Additionally, the exploration of Sp1 inhibitors could lead to novel strategies in cancer therapy. Thus, the ongoing research surrounding Sp1 recombinant protein not only aims to elucidate the intricacies of transcriptional regulation but also seeks to translate these findings into clinical applications that can improve patient outcomes in cancers and other diseases associated with Sp1 dysregulation.
