Analytical Data
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基因名
BDNF
- Application
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别名
BDNF;Brain-derived neurotrophic factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23560
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表达区间
129-247aa
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氨基酸序列
MHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQL KQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIG WRFIRIDTSCVCTLTIKRGR
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分子量
27 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Brain-Derived Neurotrophic Factor (BDNF) is a pivotal neurotrophin that plays a crucial role in the growth, maintenance, and survival of neurons. Its function is particularly important in the central nervous system, where it influences synaptic plasticity and cognitive processes such as learning and memory. Dysregulation of BDNF signaling has been implicated in various neurological and psychiatric disorders, including depression, schizophrenia, and neurodegenerative diseases like Alzheimer's. Recent research has focused on the recombinant production of BDNF to understand its mechanisms and therapeutic potential. By engineering recombinant BDNF proteins, scientists aim to dissect its structure-function relationships and explore how modifications can enhance its stability, bioavailability, and efficacy. This line of investigation also opens avenues for developing BDNF-based treatments, either through direct administration or as a part of gene therapy approaches. Understanding the biological effects of BDNF in vivo and in vitro provides valuable insights into its role in neuronal health and its potential as a target for pharmacological intervention, with the ultimate goal of improving outcomes for patients suffering from various neuropsychiatric conditions.












