Analytical Data
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Gene name
Pnlip
- Application
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Alternative Names
PL Short name: PTL Short name: Pancreatic lipase
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Species
Mouse
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P8U6
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Expression Region
17-465aa
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Molecular Weight
65.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Pnlip, or pancreatic lipase, is a crucial enzyme involved in the digestion of dietary fats by hydrolyzing triglycerides into free fatty acids and glycerol. Research on Pnlip has gained significant attention due to its role in metabolic disorders, obesity, and hyperlipidemia. The enzyme exhibits a unique structural and functional versatility, influenced by factors such as its interaction with colipase and the presence of bile salts, which enhance its activity in the gastrointestinal tract. Notably, understanding the molecular mechanisms underlying Pnlip’s function is vital for developing therapeutic strategies aimed at managing lipid metabolism disorders. Genetic variations and mutations in the Pnlip gene can lead to alterations in enzyme activity, impacting fat absorption and overall health. The recombinant production of Pnlip, particularly in heterologous systems, allows for detailed studies on its enzymatic properties and substrate specificity, providing insights into its role in lipid digestion and absorption. This research not only furthers our understanding of lipase functionality but also has potential implications for the design of enzyme inhibitors, which could serve as novel treatments for diseases associated with lipid metabolism dysregulation. Consequently, Pnlip represents a significant focus in biochemistry and nutrition research, highlighting its importance beyond basic digestive processes.
