Analytical Data
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基因名
mcr1
- Application
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别名
Polymyxin resistance protein MCR-1
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A0A0R6L508
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表达区间
1-541aa
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分子量
76.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MCR-1, or Mobilized Colistin Resistance-1, is a plasmid-mediated gene that confers resistance to colistin, an antibiotic of last resort for treating multidrug-resistant infections. Discovered in 2015, MCR-1 has raised significant concerns in public health due to its ability to spread easily among various bacteria, particularly Enterobacteriaceae. The gene encodes a phosphoethanolamine transferase that modifies the bacterial membrane, preventing colistin from effectively binding and exerting its antibacterial effects. The emergence of MCR-1 highlights the urgent need for surveillance and novel therapeutic strategies to combat antibiotic resistance. Ongoing research focuses on understanding the mechanisms of MCR-1 dissemination, its genetic context, and the evolutionary pressures that promote its adaptation. Additionally, studies aim to develop potential inhibitors of MCR-1 activity and alternatives to colistin to ensure effective treatment options for resistant infections. The significance of MCR-1 in the broader context of the global rise of antimicrobial resistance underscores the importance of interdisciplinary collaborations among microbiologists, epidemiologists, and pharmacologists in addressing this pressing health challenge.
