Analytical Data
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Gene name
MTFMT
- Application
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Alternative Names
MTFMT；FMT；FMT1；Methionyl-tRNA formyltransferase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96DP5
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Expression Region
1-389aa
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AA Sequence
MRVLVRRCWGPPLAHGARRGRPSPQWRALARLGWEDCRDSRVREKPPWRVLFFGTDQFAREALRALHAARENKEEELIDKLEVVTMPSPSPKGLPVKQYAVQSQLPVYEWPDVGSGEYDVGVVASFGRLLNEALILKFPYGILNVHPSCLPRWRGPAPVIHTVLHGDTVTGVTIMQIRPKRFDVGPILKQETVPVPPKSTAKELEAVLSRLGANMLISVLKNLPESLSNGRQQPMEGATYAPKISAGTSCIKWEEQTSEQIFRLYRAIGNIIPLQTLWMANTIKLLDLVEVNSSVLADPKLTGQALIPGSVIYHKQSQILLVYCKDGWIGVRSVMLKKSLTATDFYNGYLHPWYQKNSQAQPSQCRFQTLRLPTKKKQKKTVAMQQCIE
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Molecular Weight
43.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MTFMT (Mitochondrial Methionyl-tRNA Formyltransferase) is a crucial enzyme involved in mitochondrial protein synthesis, specifically responsible for the modification of mitochondrial tRNA. This enzyme catalyzes the formylation of methionyl-tRNA, essential for the initiation of protein synthesis in the mitochondria of eukaryotic cells. The proper functioning of MTFMT is vital for maintaining mitochondrial health and overall cellular function, as it influences the translation of mitochondrial-encoded genes, which are pivotal for oxidative phosphorylation and ATP production. Deficiencies or mutations in MTFMT can lead to a range of mitochondrial disorders, characterized by impaired energy metabolism and associated with various clinical manifestations, including neurodegenerative diseases, muscular dystrophies, and cardiomyopathies. Given the increasing recognition of the role of mitochondrial dysfunction in numerous pathologies, research into MTFMT has gained significant attention. Studies have focused on elucidating the enzyme's structure, mechanism, and regulatory pathways, alongside exploring potential therapeutic strategies to modulate its activity. Understanding MTFMT functions not only provides insights into mitochondrial biology but also paves the way for developing interventions aimed at ameliorating mitochondrial diseases and enhancing mitochondrial health. This area of research holds promise for advancing therapies that target the underlying causes of mitochondrial dysfunction and improving the quality of life for affected individuals.
