Analytical Data
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基因名
ASS1
- Application
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别名
ASS1；ASS；Argininosuccinate synthase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00966
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表达区间
1-412aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDVSREFVEEFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQVRFEL SCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DILEIEFKKG VPVKVTNVKD GTTHQTSLELFMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVLKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPTDATG FININSLRLK EYHRLQSKVT AK
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分子量
49 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ASS1 (Argininosuccinate synthase 1) is an essential enzyme involved in the urea cycle, which plays a critical role in the metabolism of nitrogen in mammals. Deficiency in the ASS1 enzyme can lead to a rare genetic disorder known as argininosuccinic aciduria, characterized by the accumulation of ammonia and argininosuccinate in the body, leading to severe neurological symptoms and developmental issues. Research on recombinant ASS1 protein focuses on understanding its structure, function, and regulatory mechanisms to develop potential therapeutic strategies for patients affected by this condition. By employing techniques such as molecular cloning, expression in various systems (e.g., bacteria, yeast, or mammalian cells), and purification methods, scientists aim to produce large quantities of functional ASS1. Furthermore, detailed studies of its enzymatic activity, interaction with other proteins, and response to metabolic changes are crucial for elucidating the pathophysiology of ASS1-related diseases. The development of recombinant ASS1 has potential applications not only in treating argininosuccinic aciduria but also in exploring the broader implications of the urea cycle in various metabolic disorders. Overall, the study of recombinant ASS1 protein provides a vital insight into nitrogen metabolism and offers a pathway towards innovative treatments for metabolic dysfunctions linked to this critical enzyme.
