Analytical Data
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Gene name
CYP27A1
- Application
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Alternative Names
CYP27A1；CYP27；Sterol 26-hydroxylase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02318
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Expression Region
1-531aa
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AA Sequence
MAALGCARLRWALRGAGRGLCPHGARAKAAIPAALPSDKATGAPGAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC
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Molecular Weight
60.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYP27A1, or cytochrome P450 27A1, is a key enzyme involved in the metabolism of cholesterol and the synthesis of bile acids, playing a crucial role in cholesterol homeostasis and cellular signaling. Mutations in the CYP27A1 gene are associated with disorders such as Smith-Lemli-Opitz syndrome and cerebrotendinous xanthomatosis, highlighting its importance in human health. Research on recombinant CYP27A1 proteins allows scientists to better understand the enzyme's structure-function relationships, enzyme kinetics, and its interactions with various substrates and inhibitors. By producing CYP27A1 in a recombinant system, researchers can generate large quantities of pure protein for biochemical assays, structural studies, and potentially for therapeutic applications. Additionally, understanding the molecular mechanisms underlying CYP27A1's role may lead to novel therapeutic strategies for cholesterol-related diseases and insights into metabolic pathways. As a result, the study of recombinant CYP27A1 not only advances basic science but also holds promise for clinical applications in treating metabolic disorders linked to cholesterol dysregulation.
