Analytical Data
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Gene name
pyrB
- Application
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Alternative Names
(Aspartate transcarbamylase)(ATCase)
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Species
Escherichia coli O45:K1
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
B7MLQ3
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Expression Region
1-311aa
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Molecular Weight
41.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of pyrB recombinant protein is rooted in the significance of the pyrimidine biosynthesis pathway in various organisms, particularly within bacteria. PyrB, a key regulatory protein encoded by the pyrimidine operon, plays a critical role in the feedback inhibition of pyrimidine nucleotide synthesis. Understanding the function and structure of PyrB can provide insights into metabolic regulation and the biological mechanisms that govern nucleotide balance, which is essential for DNA and RNA synthesis. Furthermore, the manipulation and characterization of PyrB through recombinant DNA technology have advanced our knowledge of protein functionality and regulation. By producing PyrB in a controlled laboratory setting, researchers can explore its interactions with other proteins and metabolites, offering potential applications in biotechnology and medicine, such as in the development of novel antimicrobial agents that target pyrimidine metabolism. This research also contributes to our understanding of genetic regulation and metabolic diseases, marking PyrB as an important target for further exploration in microbial physiology and genetic engineering. Overall, the study of recombinant PyrB not only enriches our fundamental knowledge of bacterial biology but also fosters the development of innovative therapeutic strategies.
