Analytical Data
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Gene name
BaP
- Application
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Alternative Names
(SVMP)(Hemorrhagic metalloproteinase BaP1)(Bap-1)
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Species
Bothrops asper
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P83512
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Expression Region
192-394aa
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Molecular Weight
30.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of BaP (benzo[a]pyrene) recombinant proteins is rooted in the need to understand the effects of polycyclic aromatic hydrocarbons (PAHs) on human health and the environment. BaP, a well-known carcinogen produced through incomplete combustion of organic materials, poses significant risks through environmental exposure, particularly in urban areas and places with industrial activity. Research has shown that BaP can induce various biological responses, including mutations and disruption of cellular processes, raising concerns about its role in cancer development. Recombinant proteins, derived from genetically engineered organisms, play a crucial role in elucidating the molecular mechanisms of BaP toxicity and its interactions with biological systems. By utilizing these proteins, scientists can investigate the metabolic pathways, enzymatic activities, and genetic responses associated with BaP exposure. Moreover, the development of recombinant protein-based assays holds potential for bioremediation efforts and the detection of BaP in environmental samples. Understanding the structure-function relationship of BaP-related proteins through recombinant technologies can facilitate the identification of biomarkers for exposure and susceptibility, contributing to public health strategies and regulatory policies aimed at minimizing the risks associated with this hazardous compound.
