Cat: PA1000-8976

Recombinant Human DBP Protein,His

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Analytical Data

  • Gene name

    DBP

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    DBP;D site-binding Protein

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P03264

  • Expression Region

    174-529aa

  • AA Sequence

    SLPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPDQVEALAAVCKTWLNEEHRGLQLTFTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEGELKCLHGSIMINKEHVIEMDVTSENGQRALKEQSSKAKIVKNRWGRNVVQISNTDARCCVHDAACPANQFSGKSCGMFFSEGAKAQVAFKQIKAFMQALYPNAQTGHGHLLMPLRCECNSKPGHAPFLGRQLPKLTPFALSNAEDLDADLISDKSVLASVHHPALIVFQCCNPVYRNSRAQGGGPNCDFKISAPDLLNALVMVRSLWSENFTELPRMVVPEFKWSTKHQYRNVSLPVAHSDARQNPFDF

  • Molecular Weight

    45.8 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

DBP (D-box binding protein) is a key regulator in various physiological processes, including cell proliferation, differentiation, and metabolic regulation. This protein functions as a transcription factor, binding to specific DNA sequences to modulate the expression of target genes. Research on DBP has gained prominence due to its involvement in circadian rhythms and its role in certain diseases, such as cancer and metabolic disorders. Understanding the structural and functional properties of DBP is vital for elucidating its mechanisms of action and regulatory pathways. Recent advancements in recombinant DNA technology have enabled the production of DBP in a controlled manner, facilitating detailed studies of its interactions with other biomolecules and its influence on gene expression. Moreover, reconstituted DBP offers potential therapeutic applications, as targeting its activity may lead to novel treatments for circadian-related disorders and other pathologies. Overall, the recombinant study of DBP provides crucial insights into its biological significance and opens up avenues for innovative research and clinical applications.

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