Analytical Data
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Gene name
EAAT3
- Application
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Alternative Names
EAAT3；EAAC1；EAAT3；HEAAC1；Excitatory amino acid transporter 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43005
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Expression Region
1-524aa
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AA Sequence
MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNLSTLEKFYFAFPGEILMRMLKLIILPLIISSMITGVAALDSNVSGKIGLRAVVYYFCTTLIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVDAMLDLIRNMFPENLVQACFQQYKTKREEVKPPSDPEMNMTEESFTAVMTTAISKNKTKEYKIVGMYSDGINVLGLIVFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDWEIFRKLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSSATLPVTFRCAEENNQVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIGQIITISITATSASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF
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Molecular Weight
57.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EAAT3, or excitatory amino acid transporter 3, is a member of the glutamate transporter family that plays a crucial role in maintaining glutamate homeostasis in the central nervous system. Elevated levels of extracellular glutamate can lead to excitotoxicity, a process implicated in various neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis. Understanding EAAT3's structure and function is essential for developing therapeutic strategies to mitigate excitotoxic damage. Research on EAAT3 has emphasized its role in regulating synaptic transmission and neural excitability, highlighting its potential as a drug target for neuroprotection. Recent advances in recombinant protein technology have enabled the production of functional EAAT3 proteins for detailed biochemical and biophysical studies. By characterizing the transport mechanism and regulatory pathways of EAAT3, researchers aim to uncover its contributions to glutamatergic signaling and identify potential mechanisms for modulating its activity in pathological conditions. This research not only enhances our understanding of synaptic physiology but also paves the way for the development of novel treatments for glutamate-related neurodegenerative diseases.
