Analytical Data
-
Gene name
EAAT2
- Application
-
Alternative Names
EAAT2；EAAT2；GLT1；；Excitatory amino acid transporter 2
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P43004
-
Expression Region
1-574aa
-
AA Sequence
MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK
-
Molecular Weight
62.1 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EAAT2 (Glutamate Transporter 2) is a crucial protein responsible for clearing extracellular glutamate, a major excitatory neurotransmitter, from the synaptic cleft in the central nervous system. Its proper function is vital for maintaining glutamate homeostasis and neuroprotection, preventing excitotoxicity that can lead to neurological disorders such as epilepsy, stroke, and neurodegenerative diseases. The human EAAT2 gene, SLC1A2, is widely expressed in astrocytes, highlighting its role in both neurotransmitter regulation and metabolic support for neurons. Given its significance in synaptic transmission and overall brain health, the structural and functional characterization of EAAT2 has garnered considerable research interest. Recombinant EAAT2 protein can be studied to elucidate its transport mechanism, regulatory pathways, and interactions with pharmacological agents. This research not only aids in understanding the physiological role of EAAT2 but also paves the way for the development of targeted therapies aimed at modulating glutamate transport to mitigate excitotoxic damage in various neurological conditions. As such, the study of recombinant EAAT2 represents a promising avenue for advancing our knowledge of neurobiology and developing potential interventions for brain disorders.
