Analytical Data
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基因名
EAAT2
- Application
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别名
EAAT2；EAAT2；GLT1；；Excitatory amino acid transporter 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43004
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表达区间
1-574aa
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氨基酸序列
MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK
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分子量
62.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EAAT2 (Glutamate Transporter 2) is a crucial protein responsible for clearing extracellular glutamate, a major excitatory neurotransmitter, from the synaptic cleft in the central nervous system. Its proper function is vital for maintaining glutamate homeostasis and neuroprotection, preventing excitotoxicity that can lead to neurological disorders such as epilepsy, stroke, and neurodegenerative diseases. The human EAAT2 gene, SLC1A2, is widely expressed in astrocytes, highlighting its role in both neurotransmitter regulation and metabolic support for neurons. Given its significance in synaptic transmission and overall brain health, the structural and functional characterization of EAAT2 has garnered considerable research interest. Recombinant EAAT2 protein can be studied to elucidate its transport mechanism, regulatory pathways, and interactions with pharmacological agents. This research not only aids in understanding the physiological role of EAAT2 but also paves the way for the development of targeted therapies aimed at modulating glutamate transport to mitigate excitotoxic damage in various neurological conditions. As such, the study of recombinant EAAT2 represents a promising avenue for advancing our knowledge of neurobiology and developing potential interventions for brain disorders.
