Analytical Data
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Gene name
sacIM
- Application
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Alternative Names
Cytosine-specific methyltransferase SacI
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Species
Streptomyces achromogenes
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O31073
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Expression Region
1-390aa
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Molecular Weight
59.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SacIM is a serine protease derived from the bacterium *Streptomyces acidiscabies*, which plays a crucial role in the bacterial life cycle and pathogenesis. The study of SacIM and its recombinant protein forms has garnered significant attention due to its potential applications in biotechnology and medicine. Understanding the structural and functional properties of SacIM can lead to insights into its mechanism of action, particularly in how it interacts with various substrates and inhibitors. Such knowledge is essential for the development of innovative therapeutic strategies, including the design of protease inhibitors that may serve as antibiotic agents against pathogenic bacteria. Additionally, the ability to produce SacIM in a recombinant form allows for high-yield production, facilitating detailed biochemical studies and the exploration of its applications in industrial processes. The research on SacIM also contributes to a broader understanding of protease enzymes, their roles in microbial physiology, and their applications in drug development and protein engineering. In summary, the research background surrounding SacIM recombinant protein involves a multifaceted approach that blends microbiology, enzymology, and applied sciences, aiming to exploit its unique properties for advancing microbial biotechnology and therapeutic interventions.
