Analytical Data
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基因名
uvsE
- Application
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别名
uvsE; BCG9842_B5482; UV DNA damage endonuclease; UV-endonuclease; UVED; EC 3.-.-.-
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种属
Bacillus cereus
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
B7IR00
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表达区间
1-317aa
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分子量
44.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the uvsE recombinant protein is rooted in its critical role in the DNA repair mechanisms within various organisms, particularly in prokaryotes such as bacteria. UvsE, an enzyme associated with the recombination repair processes, functions in the context of the RecF pathway, which is crucial for the maintenance of genomic integrity, especially under conditions of DNA damage. Understanding the structure and function of uvsE can provide insights into how cells respond to DNA damage and the mechanisms of homologous recombination, a process essential for genetic diversity and adaptation. The recombinant expression of uvsE allows for the detailed characterization of its biochemical properties and interactions within the DNA repair machinery. Furthermore, elucidating the mechanisms by which uvsE operates could have significant implications in fields such as microbiology and molecular genetics, offering potential targets for therapeutic strategies to enhance DNA repair pathways in cells and to develop innovative approaches in gene therapy. As the understanding of uvsE and its associated pathways expands, it may contribute to broader applications, including advancements in biotechnology and synthetic biology, making it a subject of substantial interest in contemporary research.












