Analytical Data
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Gene name
ACOT8
- Application
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Alternative Names
ACOT8；ACTEIII；PTE1；Acyl-coenzyme A thioesterase 8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14734
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Expression Region
1-319aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL
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Molecular Weight
38 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACOT8 (Acyl-CoA Thioesterase 8) is a member of the acyl-CoA thioesterase family, which plays a crucial role in lipid metabolism by hydrolyzing acyl-CoA esters into free fatty acids and CoA. The regulation of intracellular fatty acid levels is critical for various physiological processes, including energy homeostasis, inflammation, and cell signaling. Recent studies have highlighted the involvement of ACOT8 in key metabolic pathways and diseases such as obesity, diabetes, and certain cancers. Given its significant role in metabolic regulation, researchers are increasingly interested in characterizing ACOT8 and exploring its potential as a therapeutic target. Recombination of ACOT8 protein is essential for understanding its structure-function relationship and biochemical properties. Through recombinant expression systems, researchers can produce pure ACOT8 proteins, enabling detailed enzymatic activity assays, structural analyses, and drug screening efforts. Understanding the molecular mechanisms involving ACOT8 may provide insights into the broader implications of fatty acid metabolism in health and disease, paving the way for novel interventions in metabolic disorders.
